Col1a1^{tm6(tetO-Junb/Fos,-DsRed)Wag}

KH2 ES cells were retargeted with a "flp-in plasmid" containing a tetracycline responsive element (TRE or tetO) and a forced dimerization fusion cDNA of Junb lacking the stop codon and Fos connected by a flexible short polyGly-Ser peptide with a C-terminal FLAG-tag and an IRES DsRed reporter gene downstream of the forced dimer. A flpe-recombinase plasmid facilitated integration of the flip-in plasmid into the 3' UTR region. (J:210545)