The 14.7 kb retrotransposon insertion in intron 1 of the a (nonagouti) allele, which functionally inactivates the gene, was replaced by a neomycin and puromycin resistance cassette flanked by frt sequences. The selection cassette was excised by flp recombinase, leaving a single frt site in the intron. (J:213411)
Basic Information
Insertion, Intragenic deletion
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count