Mecp2^tm1.1Dhy

Exon 1 was replaced with a modified exon 1 in which a nucleotide substitution of A to T results in the destruction of the translation initiation site. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the modified exon 1. Western blot analysis confirmed the absence of e1 isoform expression and preserved e2 isoform expression in the brain. (J:209069)