Myf5^tm2Pas

An nLacZ gene is fused in-frame to the DNA sequences encoding the first 13 amino acids introducing a small deletion and resulting in a disruption of the gene and expression of nLacZ under the transcriptional regulation of the endogenous promoter. A TK-neomycin resistance cassette was inserted downstream of the reporter gene. In-situ hybridization analysis indicates that Myf5 and Myf6 expression is absent. (J:16491, J:33706)