Log In|Sign Up
EN
The construct consists of a 2.7 Mbp yeast artificial chromosome (YAC) sequence containing the full-length 2.3 Mbp human dystrophin gene (DMD) and a hygromycin resistance cassette on one end and a neomycin resistance cassette on the other. Spheroplast fusion of YAC-containing yeast cells with murine (DBA2 x 129OLA)F1-derived embryonic stem (ES) cells resulted in ES cells with the integration of the transgene as a tail to tail duplication into mouse chromosome 5. Analysis by Western blot and human-specific antibody demonstrated the presence of full length protein at the sarcolemma. (J:132320)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
