Cgas^{tm1c(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 78344357 of Chromosome 9 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 78345237. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the beta-geo cassette and left exon 2 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:207826)