Tg(Ube3a)1Mpan

The bacterial artificial chromosome (BAC) clone RP24-178G7 contains the entire ubiquitin protein ligase E3A locus (Ube3a) as well as ~63 kbp of 5' flanking sequence and ~21 kbp of 3' flanking sequence. DNA sequences encoding three FLAG epitope tags and two translational stop codons were inserted in-frame at the 3' coding/untranslated boundary of exon 12 of the gene on the BAC via homologous recombination/BAC recombineering. A single frt site remains just downstream of the 3xFLAG region. Two founder lines with independent integration sites were established; lines 1 and 2. Both founder lines exhibit the same expression pattern in the tissues examined (although line 2 may have slightly lower levels). Animals from line 1 were more extensively characterized. Ube3a expression was confirmed by Western blot of cortical lysates. (J:178319)