TALEN based technology was used to insert the following construct into the target gene. A loxP followed by lox2272 was inserted upstream of exon2 and its start cocon. This was followed by an FRT flanked neomycin/kanamycin construct in reverse orientation. This was followed in turn by an EGFP/Satb1 3'UTR, again in reverse orientation. Finally, a second loxP followed by lox2272 was inserted. Cre expression will invert the sequence between loxP sites allowing expression of EGFP. (J:206457)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x DBA/2)F1 x C57BL/6J
Endonuclease-mediated
Insertion
--
1
4
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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