The sequence encoding the seven N-terminal amino acids was removed and a 3xFLAG-tagg added to the C-terminal. The final allele lacks the self-excising neomycin resistance cassette inserted downstream of the modified coding region. (J:206319)
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The sequence encoding the seven N-terminal amino acids was removed and a 3xFLAG-tagg added to the C-terminal. The final allele lacks the self-excising neomycin resistance cassette inserted downstream of the modified coding region. (J:206319)