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A BAC (RP24-166N8) containing the Ltf gene was modified in bacterial cells to produce the targeting construct. A cassette containing codon-improved cre recombinase (icre), an artificial intron, the bovine growth hormone polyadenylation signal and an frt-flanked ampicillin selection marker was inserted into the first exon of Ltf. A plasmid endoding Flp was transfected into E. coli cells to delete the amp-marker. The BAC construct was verified by sequencing and microinjected into pronuclei of (B6/129Sv)F1 oocytes to produce transgenic animals. 2 lines (14 and 15) were obtained. Line 14 was chosen for further analysis. (J:210800)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
