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Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-309G21) containing the entire mouse Cspg4 (chondroitin sulfate proteoglycan 4) gene. The BAC was modified to create a membrane anchored form of EGFP (enhanced green fluorescent protein) under the control of the Cspg4 promoter followed by a polyadenylation sequence. EGFP is targeted to the plasma membrane by fusing the first 26 amino acids (containing two palmitoylation domains and a myristoylation domain) of Lck (lymphocyte-specific protein tyrosine kinase) to the EGFP N terminal. (J:209866)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
