An FRT-flanked neomycin cassette was placed in intron 4 and exons 5-7 of the gene were flanked by loxP sites. Transient expression of FLP recombinase in the ES cells excised the neomycin cassette, leaving floxed exons 5-7. (J:217860)
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An FRT-flanked neomycin cassette was placed in intron 4 and exons 5-7 of the gene were flanked by loxP sites. Transient expression of FLP recombinase in the ES cells excised the neomycin cassette, leaving floxed exons 5-7. (J:217860)