Tg(Amigo2-cre)1Sieg

The Amigo2 gene contained in a bacterial artificial chromosome (BAC; RP23-288P18) was modified with recombineering techniques so that a cre-HSV-polyA cassette(Cre recombinase sequence with an intron in the middle of the gene to enhance mammalian expression and followed by an HSV polyadenylation signal and a portion of a neo cassette) was inserted at the translation start site. This construct was injected into B6CBAF2 pronuclei and 6 founders were obtained. When crossed to Ai14 reporter mice, CA2-specific recombinase activity was not detected in offspring of any of the crosses. With injection of an adenovirus-EYFP reporter construct into the hippocampi of adult cre animals from the 6 founder lines, one line exhibited CA2-specific reporter expression and this line was used for all subsequent experiments. (J:208892)