An FRT-flanked neomycin resistance cassette was inserted downstream of exon 2. Exon 3 was replaced with a floxed exon 3-GFP fusion. Flp-mediated recombination removed the selection cassette. Western blot analysis confirmed extremely low expression levels of the fusion product in lung tissue from heterozygous mice. (J:206750)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count