Tg(hs1163-Ccre,-lacZ)1Fawa

The C-terminal fragment of cre recombinase (aa 60-343) fused to the intein-C peptide fragment (36 C terminal amino acids) of the DnaE split intein sequence was cloned into a vector containing the human enhancer 1163 sequence (hs1163) and an Hsp68 minimal promoter and lacZ reporter. The final construct included an IRES sequence separating the lacZ gene and Ccre-intein-C. Injection of the linearized construct into pronuclei was performed to generated transgenic founders. Line numbers were not identified in the publication. (J:207270)