Samhd1^{tm1d(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 156971510 of Chromosome 2 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 156972268. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the trapping cassette. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression in splenocytes. (J:202757)