The conserved WCGPC site in exon 4 was mutated to WSGPS. An FRT flanked neo-cassette was inserted downstream of exon 4 and then removed via recombinase, resulting in a single FRT site in intron 4. (J:205172)
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The conserved WCGPC site in exon 4 was mutated to WSGPS. An FRT flanked neo-cassette was inserted downstream of exon 4 and then removed via recombinase, resulting in a single FRT site in intron 4. (J:205172)