The coding sequence of exon 2 was replaced with a loxP site, lacZ gene, FRT-flanked neomycin resistance cassette, loxP site and polyadenylation sequence. Western blot analysis confirmed the absence of protein expression in the serum. (J:206120)
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The coding sequence of exon 2 was replaced with a loxP site, lacZ gene, FRT-flanked neomycin resistance cassette, loxP site and polyadenylation sequence. Western blot analysis confirmed the absence of protein expression in the serum. (J:206120)