A loxP site was inserted upstream of the 5'UTR. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette. (J:202693)
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A loxP site was inserted upstream of the 5'UTR. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette. (J:202693)