A loxP site was inserted upstream of exon 2 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Cre mediated recombination removed the neo cassette and exon 2. (J:201148)
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A loxP site was inserted upstream of exon 2 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Cre mediated recombination removed the neo cassette and exon 2. (J:201148)