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A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into intron 2 via homologous recombination, splitting intron 2 into 2 new exons (206 and 105 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
