A loxP site was inserted upstream of exon 13 and a loxP site and FRT flanked pgk-neo cassette were inserted downstream of exon 13 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:200749)
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A loxP site was inserted upstream of exon 13 and a loxP site and FRT flanked pgk-neo cassette were inserted downstream of exon 13 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:200749)