Log In|Sign Up
EN
A loxJTZ17 site was inserted at the 5' end of a cassette containing the NLS-cre-polyA sequence (nuclear localization signal-cre recombinase-polyadenylation signal). A PGK-puromycin selection cassett and a lox2272 site was inserted at the 3' end of the targeting construct. Targeted recombination in ES cells already containing Spink3tm1Kymm inserted the cre cassette upstream of exon 1 of Spink3, replacing the neo marker. Chimeras were produced from correctly targeted ES cell clones and germline transmission was established by crosses to C57BL6/J animals. (J:205182)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
