A loxP site was inserted upstream of exon 3. An FRT and loxP site were inserted downstream of exon 3. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression in E9.5 embryos. (J:203698)
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A loxP site was inserted upstream of exon 3. An FRT and loxP site were inserted downstream of exon 3. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression in E9.5 embryos. (J:203698)