Sox17^{tm4.1(icre/ERT2)Heli}

A targeting vector was designed to delete the translational stop codon of Sox17 and to introduce the codon-optimized cre fused to the triple-mutant (G400V, M543A and L544A) human estrogen receptor ligand binding domain (ERT2) sequence preceded by a viral 2A peptide sequence into exon 5. Homologous recombination in ES cells integrated the cre/ERT2 cassette and Frt-flanked neomycin marker so that the targeted Sox17 allele will produce an bicistronic mRNA that will be co-translationally cleaved into equimolar amounts of Sox17 and cre. Chimeras were crossed to a Flp deleter line to remove the neo selection marker. (J:204611)