Nr6a1^{tm1d(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 38632589 of Chromosome 2 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 38633441. The critical exon 7 is thus flanked by loxP sites. Flp-mediated recombination removed the reporter and selection cassette. Cre-mediated recombination removed exon 7. (J:204241)