Hprt1^{tm352a(Hspa1a-icre/ERT2)Ems}

The transgene was designed with an HS4 insulator region, the 878 bp Ple291 minipromoter (878 bp from a 5 prime flanking region from the mouse heat shock protein 1A locus (Hspa1a or hsp68) known to contain five HSE and to be sufficient for proper heat induction of the hsp68 gene), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. (J:270567)