Hprt1^{tm351a(Ple198-icre/ERT2)Ems}

The transgene was designed with an HS4 insulator region, the 2826 bp Ple198 minipromoter (SLC6A4-D; derived from a portion of the endogenous promoter and two upstream putative regulatory elements from the human solute carrier family 6 member 4 (SLC6A4) locus), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. (J:270567)