Timm22^{tm1b(KOMP)Wtsi}

The L1L2_gt1 cassette was inserted at position 76300011 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site was inserted downstream of the targeted exon(s) at position 76300952. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a knockout reporter construct. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)