An FRT-flanked neomycin cassette in intron 3, along with loxP sites in intron 2 and 3' of Frt site were incorporated into the genome. Flpe-mediated excision removed the neomycin cassette, leaving exon 3 floxed. (J:199365)
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An FRT-flanked neomycin cassette in intron 3, along with loxP sites in intron 2 and 3' of Frt site were incorporated into the genome. Flpe-mediated excision removed the neomycin cassette, leaving exon 3 floxed. (J:199365)