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A targeting vector constructed by BAC recombineering methods was used to swap the Notch2 intracellular domain (N2ICD) with the ICD of Notch1 (N1ICD) encoding amino acids 1,750 to 2,531, by homologous recombination in B6-derived ES cells. The 3' UTR of Notch2 was not switched so that mRNA stability could be retained. After establishment of germline transmission of this allele, the frt-flanked neo marker that had been introduced as part of the targeting vector was removed by crossing mice to flippase deleter mice. (J:198632)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
