A loxP-flanked hygromycin resistance cassette was inserted into intron 1 and a third loxP site into intron 3. Cre-mediated recombination excised the selection cassette, leaving exons 2 and 3 of the gene flanked by a pair of loxP sites. (J:119296)
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A loxP-flanked hygromycin resistance cassette was inserted into intron 1 and a third loxP site into intron 3. Cre-mediated recombination excised the selection cassette, leaving exons 2 and 3 of the gene flanked by a pair of loxP sites. (J:119296)