Setd5^tm1.1Mgn

The pRosa26.Ex1.Setd5GFP exchange vector was made on a backbone of a basal Rosa26 exchange vector which contains a 5.166 kb sequence from the Rosa26 locus, Lox71/Lox2272 sites, and flrted (flanked by FRT) Pgk-Hygro selection cassette that is used for positive selection after RMCE. A GFP-SV40 polyA sequence was inserted into exon 1 of the Setd5 gene which is located upstream of the Rosa26 exon 1 and is transcribed in the opposite orientation. Gene targeting and RMCE was performed in mESCs derived from 129S6 mice. The chimeric mice were crossed with Flpe mice to remove the hygromycin resistance gene. (J:237500)