Kdm6a^{tm1a(EUCOMM)Jae}

A targeting vector was obtained from the European knockout consortium (EUCOMM). The targeting construct contains a splice acceptor, beta-geo cassette flanked by two Flp sites preceding the third exon. In addition, the targeting construct introduced two loxP sites on either side of exon 3, allowing for conditional ablation of the exon upon the addition of cre recombinase. The flp sites flanking the splice acceptor beta-geo cassette allow for its removal after the addition of the flipase enzyme resulting in the generation of a conditional allele. (J:188485)