An FRT flanked neo cassette was inserted downstream of exon 2. Both the selection cassette and exon 2 were also flanked by loxP sites. Flp recombinase was used to excise the selection cassette from properly targeted mice. (J:199861)
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An FRT flanked neo cassette was inserted downstream of exon 2. Both the selection cassette and exon 2 were also flanked by loxP sites. Flp recombinase was used to excise the selection cassette from properly targeted mice. (J:199861)