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EN
A BAC containing the resistin (Retn) gene was modified by inserting a fragmemt encoding a nuclear localization signal (NLS) sequence, cre recombinase, and a polyadenylation signal in front of the start codon of Retn. Start sites of other genes in the BAC were mutated to prevent aberrant transcription. The purified construct was injected into fertilized C57BL/6 oocytes and founders were produced. These were crossed to wild-type and the mice were mated to ROSA26R reporter mice to assess cre activity. The line exhibiting the greatest specificity and efficiency was used for subsequent analysis. (J:199860)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
