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A targeting construct inserted in the 5' UTR in exon 1. The vector contains a loxP site, HA-tagged cDNA with (a) nucleotide substitution(s) (and complete with TGA stop codon and polyadenylation sequence), FRT-flanked neomycin resistance cassette and loxP site. The point mutation results in the amino acid substitution of isoleucine for phenylalanine at position 77 (F77I). The predicted protein product is zolpidem-insensitive. Flp-mediated recombination removed the neomycin resistance cassette. In situ hybridization confirmed reduction of transcript expression in the brain. (J:199326)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
