Exon 2 was flanked by single loxP sites. An frt flanked neomycin resistance cassette was inserted upstream of the floxed region, in intron 1. Flp-mediated recombination removed the neomycin resistance cassette and left exon 2 floxed. (J:199683)
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Exon 2 was flanked by single loxP sites. An frt flanked neomycin resistance cassette was inserted upstream of the floxed region, in intron 1. Flp-mediated recombination removed the neomycin resistance cassette and left exon 2 floxed. (J:199683)