The endogenous N-terminal Ribeye A-domain was replaced with a loxP site followed by the Ribeye A domain cDNA fused to GCaMP3 (a GFP based calcium sensor). The coding region is followed by a polyA signal, an FRT-flanked PGK-neomycin selection cassette, and a 3' prime loxP site. This knock-in allele expresses a tagged C-terminally truncated Ribeye isoform without the B-domain. (J:233453)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count