Cre-mediated cassette exchange replaces the gene trap cassette with a CAG promoter driving ubiquitous expression of the mouse full-length cDNA followed by a polyadenylation sequence and FRT-flanked puromycin resistance cassette. (J:195133)
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Cre-mediated cassette exchange replaces the gene trap cassette with a CAG promoter driving ubiquitous expression of the mouse full-length cDNA followed by a polyadenylation sequence and FRT-flanked puromycin resistance cassette. (J:195133)