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A Sleeping Beauty cargo transgene was targeted downtream of exon 2 by homologous recombination. It contained a loxP site, lacZ gene, lox2272 site, insulator from HSS4 site of chicken beta-globin locus, loxP site, FRT-flanked neomycin (neo) resistance cassette and lox2272 site. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed the lacZ and insulator and left a loxP site downstream of exon 2. (J:196292)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
