A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site followed by a FRT-flanked neomycin resistance (neo) cassette, downstream of exon 2. Flp-mediated recombination removed the neo cassette. (J:198770)
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A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site followed by a FRT-flanked neomycin resistance (neo) cassette, downstream of exon 2. Flp-mediated recombination removed the neo cassette. (J:198770)