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cDNA encoding cre recombinase was cloned into a vector containing a 5.8 kb fragment of the Myh6 (alpha-MHC) promoter including the promoter region and exons 1-3 with the hGH polyadenylation site 3' to the Myh6 fragment. The purified construct was microinjected into male pronuclei of fertilized FVB/N oocytes. 6 founders died with signs of congestive heart failure before germline transmission was established. Two founders transmitted the transgene to produce lines TG 9 and TG 12. TG9 mice developed severe cardiomyopathy and died of heart failure at 11-13 weeks. TG12 animals did not show any pathological cardiac phenotype. The TG9 line was investigated in detail. TG9 cre protein levels are much higher than in TG12 animals, and 50-100 fold higher than in Tg(Myhca-cre)1Abel mice. (J:198043)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
