Exons II, III and IV were flanked by loxP sites. An F3-flanked puromycin resistance cassette was inserted into intron IV. Mutant mice were mated with Flp transgenic animals to delete the puro marker to generated a floxed mouse line. (J:194965)
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Exons II, III and IV were flanked by loxP sites. An F3-flanked puromycin resistance cassette was inserted into intron IV. Mutant mice were mated with Flp transgenic animals to delete the puro marker to generated a floxed mouse line. (J:194965)