Dlg4^tm1.1Tdoi

Exons 5 through 8 were replaced with a modified full-length cDNA and EGFP fusion followed by a floxed neomycin resistance cassette. The modified cDNA contains the mutations S78N (AGC to AAC), A80V (GCA to GTA), S183N (AGC to AAC), and A185V (GCA to GTA), and the deletions T83 to I88 and V178 to I183 that mutate PDZ1/2 to resemble PDZ3. Cre-mediated recombination removed the neomycin resistance cassette. (J:197875)