An FRT-neo-FRT-loxP cassette was introduced to intron 1, and a second loxP site was place 5' of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. QRT-PCR confirmed the absence of transcript expression in embryos. (J:198115)
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An FRT-neo-FRT-loxP cassette was introduced to intron 1, and a second loxP site was place 5' of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. QRT-PCR confirmed the absence of transcript expression in embryos. (J:198115)