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The vector was made on a backbone of a basal construct containing 5.166 kb from Rosa26 region that was absent in the Rosa26LCA allele, tandem Lox71/Lox2272 sites and a flrted (flanked by FRT)Pgk-Neo cassette for positive selection of ES cells after RMCE. Within the Rosa26 gene sequences the region from -228 to +81 were replaced by a Wnt response element (7xTcf/Lef binding site) fused to a TATA-Cerulean (CFP) reporter. The chimeric mice were crossed with Flpe mice to remove the neomycin resistance gene. (J:195112)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
