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A cassette encoding an IRES-tTA (tetracycline transactivator) and tetO-cre was inserted into the 3' UTR of the Rax (Rx) gene, 104 bp from the stop codon. A neo resistance marker was included in the cassette. The construct was electroporated into ES cells for homologous recombination. Correctly targeted germline mutants were crossed to flpe-expressing transgenic mice to remove the neo marker. Further breeding to wild-type mice removed the flpe transgene. Removal of doxycycline from the animals' diets permits Rax-dependent cre expression. (J:195568)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
