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A hygromycin selection cassette and DR-GFP reporter, were inserted into the locus via homologous recombination. The DR-GFP reporter consists of , consisting of two mutated GFP genes, SceGFP, which is disrupted at an LweI restriction site by the 18-bp recognition site for the I-SceI endonuclease, and iGFP, which is truncated at both the 5' and 3' ends. Following homology directed repair a functional GFP gene expressed under the control of a beta-actin promoter and CMV enhancer is generated. (J:194229)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
