By homologous recombination, a targeting construct replaced the endogenous exon 1, intron, and exon 2 of Egr3 with a cassette encoding nuclear-localized cre recombinase with and a PGK-neo selection marker flanked with forward-oriented Frt sites. The neo was excised from the germline by mating mice to CAG-FLPe mice. (J:196371)